Micropipette aspiration of cultured bovine aortic endothelial cells exposed to shear stress.

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Micropipette aspiration of cultured bovine aortic endothelial cells exposed to shear stress.

The mechanical properties of cultured bovine aortic endothelial cells exposed to a fluid-imposed shear stress were studied using the micropipette technique. The cells, which were attached to a Thermanox plastic substrate, were exposed to a specific steady shear stress of either 10, 30, or 85 dynes/cm2 and for a duration ranging from 0.5 to 24 hours. Morphological changes in shape and orientatio...

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Micropipette aspiration of living cells.

The mechanical behavior of living cells is studied with micropipette suction in which the surface of a cell is aspirated into a small glass tube while tracking the leading edge of its surface. Such edges can be tracked in a light microscope to an accuracy of +/-25 nm and suction pressures as small as 0.1-0.2 pN/microm2 can be imposed on the cell. Both soft cells, such as neutrophils and red cel...

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ژورنال

عنوان ژورنال: Arteriosclerosis: An Official Journal of the American Heart Association, Inc.

سال: 1987

ISSN: 0276-5047

DOI: 10.1161/01.atv.7.3.276